Composite

Part:BBa_K3906000:Design

Designed by: Ruyi Shi   Group: iGEM21_ASTWS-China   (2021-10-14)


PETase-SpyCatcher


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 195


Design Notes

For strong PETase expression, PETase was inserted into the pET-21a(+) vector and we did codon optimization for successful E. coli expression. In order to make PETase and MHETase effectively coupled, SypCatcher and SpyTag system was used.


Source

The sequence was based on BBa_K3576001.

References